SrasV1, Main, Exploration, bibRecord, 004E76

Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

Identifieur interne : 004E76 ( Main/Exploration ); précédent : 004E75; suivant : 004E77

Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

Auteurs : Cheng-Wen Lin [Taïwan] ; Fuu-Jen Tsai [Taïwan] ; Chang-Hai Tsai [Taïwan] ; Chien-Chen Lai [Taïwan] ; LEI WAN [Taïwan] ; Tin-Yun Ho [Taïwan] ; Chang-Chi Hsieh [Taïwan] ; Pei-Dawn Lee Chao [Taïwan]

Source :

Antiviral research [ 0166-3542 ] ; 2005.

RBID : Pascal:05-0472143

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Peptidases, Racine, Antiviral, Origine végétale, Cruciferae, Phénols, Isatis indigotica.

English descriptors

KwdEn :
- Antiviral, Cruciferae, Peptidases, Phenols, Plant origin, Root, Severe acute respiratory syndrome virus.

Abstract

The 3C-like protease (3CL^pro) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL^pro effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL^pro demonstrated that IC₅₀ values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC₅₀: 217 μM) was more efficient in blocking the cleavage processing of the 3CL^pro than indigo (IC₅₀: 752 μM) and beta-sitosterol (IC₅₀: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL^pro, in which the IC₅₀ was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.

Affiliations:

Taïwan

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Le document en format XML

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<front><div type="abstract" xml:lang="en">The 3C-like protease (3CL<sup>pro</sup>
) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL<sup>pro</sup>
 effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL<sup>pro</sup>
 demonstrated that IC<sub>50</sub>
 values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC<sub>50</sub>
: 217 μM) was more efficient in blocking the cleavage processing of the 3CL<sup>pro</sup>
 than indigo (IC<sub>50</sub>
: 752 μM) and beta-sitosterol (IC<sub>50</sub>
: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL<sup>pro</sup>
, in which the IC<sub>50</sub>
 was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.</div>
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<affiliations><list><country><li>Taïwan</li>
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<name sortKey="Lin, Cheng Wen" sort="Lin, Cheng Wen" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
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<name sortKey="Tsai, Fuu Jen" sort="Tsai, Fuu Jen" uniqKey="Tsai F" first="Fuu-Jen" last="Tsai">Fuu-Jen Tsai</name>
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Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri